Coupling of proteolytic quenching and HPLC to enzyme reactions
Summary
Type: HPLC Application #: 107220
Detection: UV, 254nm Conditions: eluent: linear gradient (dotted line) from 0.1 to 0.6M ammonium acetate solution pH 5.5 flow rate 1.0ml/min Authors: Guasch A Source: J Chromatogr 473 (1989) 281 - 286
Details
Legend: Fig. 1. Chromatography of the components of a reaction mixture (40mM C>p, 0.6
µM RNase A) incubated for 5min, stopped by addition of H3PO4 - HCl and then
pepsin.